2013-05-25T23:11:46Z http://repub.eur.nl/oai

oai:repub.eur.nl/res/pub/10377 2013-03-29T14:10:32Z org:9805 org:9755 org:9833 org:9746 access:open type:article

urn:NBN:nl:ui:15-1765/10377 2013-03-29T14:10:32 mods http://hdl.handle.net/1765/10377 hdl:1765/10377 urn:NBN:nl:ui:15-1765/10377 Threonine utilization is high in the intestine of piglets text article http://hdl.handle.net/1765/10377 http://repub.eur.nl/res/pub/10377/15795432.pdf application/pdf Filesize: 126 KB born digital The whole-body threonine requirement in parenterally fed piglets is substantially lower than that in enterally fed piglets, indicating that enteral nutrition induces intestinal processes in demand of threonine. We hypothesized that the percentage of threonine utilization for oxidation and intestinal protein synthesis by the portal-drained viscera (PDV) increases when dietary protein intake is reduced. Piglets (n = 18) received isocaloric normal or protein-restricted diets. After 7 h of enteral feeding, total threonine utilization, incorporation into intestinal tissue, and oxidation by the PDV, were determined with stable isotope methodology [U-(13)C threonine infusion]. Although the absolute amount of systemic and dietary threonine utilized by the PDV was reduced in protein-restricted piglets, the percentage of dietary threonine intake utilized by the PDV did not differ between groups (normal protein 91% vs. low protein 85%). The incorporation of dietary threonine into the proximal jejunum was significantly different compared with the other intestinal segments. Dietary, rather than systemic threonine was preferentially utilized for protein synthesis in the small intestinal mucosa in piglets that consumed the normal protein diet (P < 0.05). Threonine oxidation by the PDV was limited during normal protein feeding. In protein-restricted pigs, half of the total whole-body oxidation occurred in the PDV. We conclude that, in vivo, the PDV have a high obligatory visceral requirement for threonine. The high rate of intestinal threonine utilization is due mainly to incorporation into mucosal proteins Schaart, M.W. Schaart Maaike aut Schierbeek, H. Schierbeek Henk aut Schoor, S.R. van der Schoor, van der Sophie aut Stoll, B. Stoll B. aut Burrin, D.G. Burrin Douglas aut Reeds, P.J. Reeds P.J. aut Goudoever, J.B. van Goudoever, van Hans aut 298688441 298693216 11060993X Erasmus MC: University Medical Center Rotterdam Supporting host sht en English The Journal of Nutrition 0022-3166 2005-01-01 Animals Research Support, Non-U.S. Gov't Swine Research Support, U.S. Gov't, Non-P.H.S. Carbon Isotopes Enteral Nutrition Intestinal Mucosa/metabolism Intestines/physiology Portal System/physiology Threonine/blood/*metabolism Erasmus University Rotterdam oai:repub.eur.nl/res/pub/10377 IR OAIPMH feed (http://repub.eur.nl/oai) 2007-06-26T07:37:24Z 2011-11-23T09:59:13Z http://purl.org/eprint/accessRights/OpenAccess