2013-06-19T06:54:02Z http://repub.eur.nl/oai

oai:repub.eur.nl/res/pub/29120 2013-01-22T12:50:14Z org:9830 org:9755 org:9816 org:9746 access:open type:article

urn:NBN:nl:ui:15-1765/29120 2013-01-22T12:50:14 mods http://hdl.handle.net/1765/29120 hdl:1765/29120 urn:NBN:nl:ui:15-1765/29120 doi:10.1038/hdy.2008.18 Heterozygosity and lungworm burden in harbour seals (Phoca vitulina) text article http://hdl.handle.net/1765/29120 http://dx.doi.org/10.1038/hdy.2008.18 text/html born digital In several studies, heterozygosity measured at around 10 microsatellite markers correlates with parasite load. Usually the effect size is small, but while this may reflect reality, it may also be possible that too few markers are used or the measure of fitness contains too much error to reveal what is actually a much stronger underlying effect. Here, we analysed over 200 stranded harbour seals (Phoca vitulina) for an association between lungworm burden and heterozygosity, conducting thorough necropsies on the seals and genotyping the samples obtained for 27 microsatellites. We found that homozygosity predicts higher worm burdens, but only in young animals, where the worms have the greatest impact on fitness. Testing each locus separately, we found that a significant majority reveal a weak but similar trend for heterozygosity to be protective against high lungworm burden, suggesting a genome-wide effect, that is, inbreeding. This conclusion is supported by the fact that heterozygosity is correlated among markers in young animals but not in otherwise equivalent older ones. Taken as a whole, our results support the notion that homozygosity increases susceptibility to parasitic infection and suggest that parasites can be effective in removing inbred individuals from the population. Rijks, J.M. Rijks J.M. aut Hoffman, J.I. Hoffman J.I. aut Kuiken, T. Kuiken Thijs aut Osterhaus, A.D.M.E. Osterhaus Ab aut Amos, W. Amos W. aut 29868697X 074960172 Erasmus MC: University Medical Center Rotterdam Supporting host sht en English Heredity 0018-067X 100 6 587 593 2008-06-23 Harbour seal (Phoca vitulina) Heterozygosity-fitness correlation (HFC) Lungworm burden Otostrongylus circumlitus Parafilaroides gymnurus Erasmus University Rotterdam oai:repub.eur.nl/res/pub/29120 IR OAIPMH feed (http://repub.eur.nl/oai) 2011-11-22T15:26:45Z 2011-12-20T14:59:12Z http://purl.org/eprint/accessRights/OpenAccess