2013-05-20T04:14:10Z http://repub.eur.nl/oai

oai:repub.eur.nl/res/pub/9210 2012-11-14T12:37:14Z org:9830 org:9755 org:9821 org:9746 access:open type:article

urn:NBN:nl:ui:15-1765/9210 2012-11-14T12:37:14 mods http://hdl.handle.net/1765/9210 hdl:1765/9210 urn:NBN:nl:ui:15-1765/9210 The prevalence and clonal expansion of high-level gentamicin-resistant enterococci isolated from blood cultures in a Dutch university hospital text article http://hdl.handle.net/1765/9210 http://repub.eur.nl/res/pub/9210/10590281.pdf application/pdf Filesize: 122 KB born digital We studied the prevalence and clonality of high-level gentamicin-resistant enterococci (HLGRE) in a Dutch university hospital. Of 238 enterococcal strains isolated from blood cultures between 1991 and 1997, 57 were HLGRE. Genomic analysis of these strains revealed 19 different genotypes, two of which were encountered more frequently [type A (12/57), type B (23/57)]. The spread of these types largely explained the rise in HLGRE incidence from 14% in 1991 to 31% in 1997. However, the contribution of unique strains to the total HLGRE burden also increased from 4% to 16%. We conclude that both clonal expansion and the emergence of unique HLGRE have contributed significantly to the increasing incidence of HLGRE. Braak, N.P.W.C.J. van den Braak, van den Nicole aut Belkum, A.F. van Belkum, van Alex aut Kreft, D. Kreft D. aut Witt, R. te Witt, te René aut Verbrugh, H.A. Verbrugh Henri aut Endtz, H.P. Endtz Hubert aut 073664952 068402856 10770420X Erasmus MC: University Medical Center Rotterdam Supporting host sht en English Journal of Antimicrobial Chemotherapy 0305-7453 1999-01-01 Humans Electrophoresis, Gel, Pulsed-Field Polymerase Chain Reaction/methods Microbial Sensitivity Tests Anti-Bacterial Agents/*pharmacology Culture Media Hospitals, University Bacteremia/epidemiology/microbiology DNA, Bacterial/analysis Drug Resistance, Microbial/genetics Enterococcus/*drug effects/*genetics/isolation & purification Gentamicins/*pharmacology Gram-Positive Bacterial Infections/*epidemiology/*microbiology Erasmus University Rotterdam oai:repub.eur.nl/res/pub/9210 IR OAIPMH feed (http://repub.eur.nl/oai) 2007-03-20T14:07:31Z 2011-11-23T09:59:13Z http://purl.org/eprint/accessRights/OpenAccess