ࡱ > | z x v t r ` e bjbj .y j j 7 l % % % T % v v v Bw w % h
y z L B{ B{ B{ OB
OB
OB
ϊ
ъ
ъ
ъ
ъ
ъ
ъ
$
0
T
- % OB
0[ OB
OB
OB
L
B{ B{ k "
L
L
L
OB
f B{ % B{ ϊ
L
OB
ϊ
L
" L
S
|
%
B{ y `umo}% P v F
~
,
D 8
0 h
~
F
L
% %
Title:
Shortening induced effects on force (re)development in pig urinary smooth muscle
Authors:
E. van Asselt, J.J.M. Pel and R. van Mastrigt
Affiliation:
Department of Urology, sector Furore, Erasmus MC, Rotterdam, The Netherlands
Running head:
Shortening induced changes in bladder contractility
Correspondence to:
Els van Asselt
Department of Urology, sector Furore, room Ee 1630
Erasmus MC
PO Box 1738
3000 DR Rotterdam
The Netherlands
tel: +31-10 - 4088057
fax: +31-10 - 4089451
HYPERLINK "mailto:e.vanasselt@erasmusmc.nl" e.vanasselt@erasmusmc.nl
word count: 3226
List of symbols:
Fiso maximum force of isometric contraction at stop length
Fmax maximum force of isometric contraction at start length
Fmin minimum force at the end of shortening at stop length
Fred maximum redevelopment force after shortening at stop length
F1,2 amplitudes of bi-exponential redevelopment force fitted to Fred
Fsid measure of shortening induced deactivation: Fred /Fiso
lshort shortening amplitude
lstart start length of the muscle bundle (lstop + (4x vshort))
lstop stop length of the muscle bundle (200% of lslack)
lslack length of muscle bundle at which slight lengthening results in force of 150 (N
t time
t0 the delay time between activation of stimulation field and force generation
tdelay the duration of the stimulation before shortening
tint period during which stimulation is interrupted immediately after shortening
(mono time constant of mono-exponential force development
(1,2 time constants of bi-exponential force development
qmono,bi sum of the absolute differences between measured and fitted force values
divided by the sum of the measured force values for respectively the mono-
and bi-exponential fit
vshort shortening velocity
ABSTRACT
Introduction: When muscle is allowed to shorten during an active contraction, the maximum force that redevelops after shortening is smaller than the isometric force at the same muscle length without prior shortening. We studied the course of force redevelopment after shortening in smooth muscle to unravel the mechanism responsible for this deactivation.
Method: In a first series of measurements the shortening velocity was varied resulting in different shortening amplitudes. In a second series, the duration of stimulation before shortening (shortening delay) was varied. In a third series the stimulation was interrupted for a certain duration immediately after shortening. Force, muscle length and stimulation were continuously recorded. Time constants were calculated to describe the rate of force development before and after shortening.
Results: With increasing shortening amplitude and with increasing shortening delay, force redevelopment decreased. Redevelopment increased with an increase in the interruption time. After stimulus interruption force redeveloped mono-exponentially with a time constant similar to that of isometric contractions (~ 3 s). Without the interruption of stimulation, the redevelopment of force immediately after shortening was best described by two time constants; one similar to and one about 3-5 times faster than the isometric time constant.
Discussion: Force (re)development is caused by a cascade of events leading to the cycling of cross-bridges. In smooth muscle, isometric force development is described by a time constant of about 3 s. Force redevelopment immediately after shortening involves a second process which takes place at a faster rate (time constant about 1 s). We assume that this process is faster due to the immediate availability of cytoplasmic calcium released during active shortening. Deactivation presumably is caused by disorganisation of filaments during shortening.
Key words: contraction; deactivation; stoptest; time constant
INTRODUCTION
When muscle is allowed to shorten during an active contraction, the maximum force that redevelops after shortening is smaller than the isometric force at the same muscle length without prior shortening. This phenomenon has been called force depression, force deficit and shortening induced deactivation. It has been described in striated ADDIN EN.CITE de Ruiter1998140de Ruiter, CJde Haan, AJones, DASargeant, AJ1998Shortening-induced force depression in human adductor pollicis muscleJ Physiol5072583 - 591(Herzog and Leonard, 1997; de Ruiter et al., 1998), heart ADDIN EN.CITE Suga1977500Suga, H.Yamakoshi, K1977Effects of stroke volume and velocity of ejection on end-systolic pressure of canine left ventricle.End-systolic volmue clamping.Circulation Res40445 - 450Colomo1994130Colomo, FPoggesi, CTesi, C1994Force responses to rapid length changes in single intact cells from frog heartJ Physiol4752347 - 350(Colomo et al., 1994; Suga and Yamakoshi, 1977) and smooth muscle ADDIN EN.CITE Gunst19865039422040002-95132501 Pt 11986JanEffect of length history on contractile behavior of canine tracheal smooth muscleC146-54Gunst, S. J.Am J PhysiolAcetylcholine/pharmacologyAnimalDogsElectric Stimulation*Muscle ContractionMuscle, Smooth/*physiologySupport, Non-U.S. Gov'tSupport, U.S. Gov't, P.H.S.Time FactorsTrachea/*physiologyhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=3942204(Gunst 1986; Gunst et al., 1993; Meiss 1993) and was found to depend both on shortening amplitude and shortening velocity.
Isometric force development in (smooth) muscle cells is caused by cross-bridge formation (actin myosin) induced by an increase in the concentration of intracellular calcium. One of the hypotheses put forward to explain shortening induced deactivation involves the (re)organization of filaments ADDIN EN.CITE Gunst199519077626210002-95132685 Pt 11995MayMechanisms for the mechanical plasticity of tracheal smooth muscleC1267-76Department of Physiology and Biophysics, Indiana University School of Medicine, Indianapolis 46202, USA.Gunst, S. J.Meiss, R. A.Wu, M. F.Rowe, M.Am J PhysiolAnimalBiomechanicsDogsElectric StimulationIsometric ContractionMuscle, Smooth/*physiologySupport, U.S. Gov't, P.H.S.Trachea/*physiologyhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7762621(Gunst et al., 1995; Gunst et al., 1993; Herzog and Leonard 1997; Marechal and Plaghki, 1979; Pratusevich et al., 1995; Shen et al., 1997). At rest, the cross-bridge forming filaments actin and myosin are arranged in a well-organized pattern with overlapping zones and the actin filaments are anchored to the cell membrane. The actin cytoskeleton can adapt to changes in muscle length but the rate of a structural (re)arrangement in smooth muscle is limited (Gunst et al., 1995; Gunst and Wu, 2001; Mehta et al., 1996). Presumably, during active shortening, the contractile filaments become disorganized, and larger shortening amplitudes and/or shortening velocities result in more disorganization. In striated muscle, a short interruption of stimulation after active shortening allows a full recovery of force, presumably because all cross-bridges detach and actin and myosin form new bridges again ADDIN EN.CITE Morgan2000430Morgan, D.L.Whitehead, N.P.Wise, A.K.Gregory, J.E.Proske, U.2000Tension changes in the cat soleus muscle following slow stretch or shortening of the contracting muscleJ Physiol5223503 - 513de Ruiter1998140de Ruiter, CJde Haan, AJones, DASargeant, AJ1998Shortening-induced force depression in human adductor pollicis muscleJ Physiol5072583 - 591(Morgan et al., 2000; de Ruiter et al., 1998).
In a pilot study using the stoptest, we found that shortening amplitude was the key factor controlling the degree of deactivation in pig urinary bladder muscle strips ADDIN EN.CITE Van Asselt2002410Van Asselt, E.Pel, J.J.M.van Mastrigt, R.2002Shortening induced deactivation in pig urinary smooth muscleJ Muscle Res Cell Mot23137(van Asselt et al., 2002). During the stoptest, muscle is shortened from one preset length to another while being stimulated. This test has the advantage that the forces at different shortening velocities are measured at fixed lengths and correction for passive forces is possible. Especially in smooth muscle where passive forces are relatively high, this correction is indispensable. We studied the course of force redevelopment after shortening in urinary bladder smooth muscle in three series of measurements in which respectively the shortening amplitude, the stimulation duration before shortening (shortening delay) and the duration of an interruption of stimulation after shortening (stimulus interruption) were varied. We hypothesize that deactivation is caused by a combination of disorganization of myofilaments, (re)attachment of cross-bridges and high cytoplasmic calcium concentration after shortening and that full recovery is only possible when all cross-bridges detach. In series 1, active shortening evoked a decrease in force. A larger shortening amplitude results in a larger force decrease and more disorganized filaments. The subsequent redevelopment of force is related to the shortening amplitude applied. In series 2, the shortening amplitude was fixed but the stimulation duration before shortening was varied. We assume that the longer this duration, the more cross bridges attach before shortening, the more cross bridges detach during shortening and the higher the cytoplasmic calcium concentration after shortening. We suggest that in both series the high cytoplasmic calcium concentration after shortening interferes with the reattachment of cross-bridges in the redevelopment phase. In series 3, the interruption of the stimulus not only enabled detachment of eventually all cross-bridges but also allowed uptake of calcium by the sarcoplasmic reticulum and thus a better recovery. The time course of force (re)development was described mathematically with mono- and bi-exponential functions, to identify different processes at different time scales that could be related to the physiological parameters mentioned above.
MATERIALS AND METHODS
Tissue handling: preparation and stimulation
Urinary bladders of freshly killed pigs were collected from the local slaughterhouse or from pigs sacrificed in the department of cardiology, Erasmus MC. Bladders were stored in cold modified Krebs solution (118 mM NaCl; 4.7 mM KCl; 25 mM NaHCO3; 1.2 mM KH2PO4; 1.8 mM CaCl2; 1.2 mM MgSO4 and 11 mM glucose aerated with 95% O2 and 5% CO2 (pH 7.4)). Two strips of about 20 x 20 mm were cut from the antero-lateral wall of each bladder. One strip was kept overnight in 4 C Krebs solution to be used the next day. A small muscle bundle (~ 0.7 x 3 mm), in which the muscle cells were oriented in the longitudinal direction, was cut from the serosal side of the other strip and mounted in an organ bath filled with Krebs (refresh rate of 1 ml hr-1). The temperature of the Krebs was kept constant at 37 C by infrared radiation. One side of the bundle was clamped in a pair of tweezers connected to a length controller ADDIN EN.CITE van Koeveringe1997402van Koeveringe, G.A.1997Dynamics of smooth muscle contractionDepartment of UrologyRotterdamErasmus University181(van Koeveringe, 1997) and the bundle was electrically stimulated to attain the smallest physiological length. Then, the other side of the bundle was clamped in a second pair of tweezers connected to a KG3 force transducer (resolution 2 N) linked to a BAM3 amplifier (Scientific Instruments, Heidelberg, Germany). After a rest period of 10 minutes, the bundle was gently stretched to a slack length, lslack, defined as the length at which a small increase in length resulted in an increase in passive force of ~150 (N. Thereafter the muscle bundle was stretched to a stop length, lstop set at 200% of slack length and further stretched to a start length lstart. The muscle bundle was stimulated using an electrical field generated between two platinum electrodes (8V, 125Hz and 5ms pulse width). The length controller was used to shorten the muscle bundle from lstart to lstop with a constant shortening velocity. Previous measurements in bladder muscle showed that the maximum isometric force was reached at muscle lengths of 290 ( 68% of slack length ADDIN EN.CITE Minekus2001390Minekus, J.van Mastrigt, R.2001Length dependence of the contractility of pig detrusor smooth muscle fibresUrol Res29126 - 133(personal observation, Minekus and van Mastrigt, 2001). The measurements presented here were thus performed close to optimum length (start length = 200% of slack length + 5-35% stretch).
Measurement protocol
After two isometric test stimulations with a 10 minutes interval, each bundle was subjected to a standard stoptest protocol (Fig. 1). The clamped bundle was slowly stretched to a start length, lstart. This length was the sum of lstop and the product of the programmed shortening duration and shortening velocity, vshort. The difference lstart-lstop was called the shortening amplitude, lshort. One minute after lengthening, the passive response of the muscle (no electrical stimulation) to the programmed shortening was recorded. One minute after restretching to lstart, the procedure was repeated with the electrical stimulation switched on. In this way, the total force response measured during field stimulation could be corrected by subtracting the corresponding passive force response measured without stimulation. The result was called the active force. Upon stimulation, with a total duration of 30 s, the active force at lstart increased to a maximum value, Fmax. During the shortening from lstart to lstop, force decreased to a minimum value Fmin. After stopping the shortening at lstop force redeveloped to Fred. Five minutes later, the isometric force Fiso was measured at lstop. After 10 minutes the whole protocol was repeated.
Series 1:
Twenty muscle bundles from 13 bladders were subjected each to a vshort of subsequently 25, 100, 75, 175 and 50 (m s-1 resulting in an lshort of 100, 400, 300, 700 and 200 (m. The bundles were shortened during 4 s, 14 s after the beginning of stimulation (Fig. 2A).
Series 2:
Seven muscle bundles from 4 bladders were stimulated each and shortened during 4 s with a shortening velocity of 100 (m s-1 respectively 14, 11, 8, 5, 3 or 2 s after the onset of stimulation (shortening delay tdelay) (Fig. 2B)
Series 3:
After 14 s of stimulation, nineteen muscle bundles from 17 bladders were shortened during 4s at 100 (m s-1. Immediately after the shortening, the stimulation was interrupted for 2, 3, 4, 6, 12 or 22 s (interruption time tint). Strips were subjected to 2-4 different interruption times in random order (Fig. 2C).
Data processing
All measurements were analyzed using self-written programs (Matlab(). The active redevelopment force divided by the active isometric force at the same muscle length: Fred/Fiso = Fsid was considered a measure for shortening induced deactivation (a low value of Fsid signifies a large deactivation). Herzog et al., in the soleus muscle of the cat, used the difference of redevelopment and isometric force as a measure for shortening induced deactivation (Herzog et al., 2000; Corr and Herzog, 2005; Leonard and Herzog, 2005). For reasons of comparison we also calculated correlation coefficients using Fred-Fiso (see Table I and II). The decrease of force during shortening was described by Fmax Fmin (see also Fig. 1).
The active force development before shortening, after shortening, and 5 min later when the muscle was isometrically restimulated were fitted with a mono- and a bi-exponential function by minimizing the absolute difference between measured and estimated force values.
A. mono-exponential function:
EMBED Equation.3 (eq. 1)
F0 is the maximum extrapolated force, t the time, t0 the delay time between activation of stimulation field and force generation and (mono the time constant of the contraction
B. bi-exponential function:
EMBED Equation.3 (eq. 2)
F1 and F2 are the two amplitudes of force development and (1 and (2 the time constants of the contraction.
The relative contributions of F1 and F2 to the active force were expressed as: %Fn = 100* Fn/(F1+F2) with n = 1 , 2 (Fig. 3). The reliability of the mono- and bi-exponential fits was quantified by the fit error, the sum of the absolute differences between measured and fitted force values divided by the sum of the measured force values (respectively qmono and qbi). When F1,2 was negative or when qbi/qmono>1, the bi-exponential fit was considered inappropriate and only the mono-exponential fit was taken into consideration.
An estimate for the amount of work performed by the muscle during shortening (W) was derived by calculating the area under the force curve during shortening (F x lshort, see hatched area in Fig. 1) (Herzog et al., 2000).
Statistical analysis
Linear regression analysis and Spearmans correlation test were used to test the relationship beween shortening amplitude, shortening delay, interruption time and deactivation, and Fmax-Fmin and to correlate deactivation with Fmax-Fmin, work, %F1 and F1/F2. Fmax-Fmin from series 1 and 2 were compared with the Mann-Whitney test. The time constants (mono and (1 and also (1 and (2 of the redevelopment force were pairwise tested with Students t test. The redevelopment force and time constants of muscle strips used on day 0 and day 1 were compared with the Students t test.
All analyses were performed using SPSS(. Results were considered significant if p < 0.01.
Experiments were carried out as outlined in the Erasmus University of Rotterdam Guidelines for the Care and Use of Laboratory Animals, which in general follows the NIH Guide for the Care and Use of Laboratory Animals. The use of bladders obtained from the department of Cardiology was approved of by the METC (medical ethical committee of the University) (the use of slaughterhouse material does not require formal approval).
RESULTS
Three bladders were discarded because the bundles did not contract upon electrical stimulation. Since there were no significant differences in force development between muscle bundles from bladders on the day of excision and one day later, all data were pooled.
Force (Table I and II)
Table I summarizes the relationships between shortening amplitude, shortening delay, stimulus interruption and force.
Fred/Fiso significantly decreased with an increase in lshort, an increase in tdelay and a decrease in tint.
Fred Fiso did not depend on lshort but significantly decreased with tdelay and significantly increased with tint. Fmax-Fmin significantly increased with lshort and tdelay and did not depend on tint. The mean Fmax-Fmin was significantly larger in series 1 than in 2 (Mann-Whitney test).
Work significantly increased with tdelay but did not depend on lshort or tint.
Table II summarizes the relationships between deactivation and decrease of force during shortening, work, %F1 and F2/F1.
F1 represented 82 ( 7 % of the total recovery force (series 1 and 2) and F1/F2 was 0.31 ( 0.16 in series 1 and 0.26 ( 0.14 in series 2. These parameters were not related to lshort, tdelay or tint.
Time constants (Table III)
Isometric contractions at lstart and lstop and contractions after shortening with an interruption of the stimulus were best fitted with a mono-exponential function ((mono). Force redevelopment curves immediately after shortening without interruption were best fitted with a bi-exponential function and (1 was significantly larger than (2 (paired t-test). The values for (1 and (2 in series 3 were considered unreliable because of the small number of measurements remaining after applying the exclusion criteria.
DISCUSSION
We studied shortening induced deactivation in the pig urinary bladder with special attention to the time course of force (re)development. We hypothesize that deactivation is a result of the disorganization of the myofilaments and changes in intracellular calcium concentration. Presumably, cross bridges detach during active shortening because the force decreases. We suggest that this decrease in combination with the length decrease of the muscle fibers results in disorganization of the myofilaments and that the extent of the force decrease is a measure for disorganization. The force decrease during shortening (Fmax-Fmin) was significantly smaller in series 2 than in series 1 whereas the deactivation was only somewhat smaller. From these results we conclude that deactivation is not caused by disorganization only. In the literature it has been reported that the intracellular calcium concentration is related to the shortening length of muscle fibers (Gunst, 1989) and that the affinity of myofilaments for calcium can change (Leach et al, 1999). The larger the shortening amplitude, the larger the force decrease during shortening, the higher the cytoplasmic calcium concentration. Presumably, this free calcium can be sequestered extracellularly and/or into the sarcoplasmic reticulum via the usual mechanisms but alternatively it could also be used immediately for the redevelopment of force (a process described by the fastest time constant). In series 2, the stimulation duration is reduced and therefore, less myofilaments become involved in the production of force. Consequently, less filaments are affected during shortening, the amount of released calcium is smaller and the affinity for calcium of a smaller number of filaments is affected, therefore, after shortening more filaments are able to develop force and deactivation is less. In series 3, reorganization of myofilaments is facilitated by interruption of the electrical stimulus; the force decrease continues during the interruption resulting in more detached cross bridges which enables the formation of more new ones.
The differences in correlation between Fred/Fiso and Fred-Fiso and the various parameters in Table I and II are most likely caused by the two definitions. We used the quotient Fred/Fiso because this is a relative measure, independent of the strength (muscle mass) of the individual muscle bundles. The fact that in series 1, W was significantly correlated to Fred-Fiso but not to Fred/Fiso (and vice versa for lshort) suggests that this correlation may be artefactual and caused by variations in muscle mass that influence both W and Fred-Fiso but not Fred/Fiso. In series 2 and 3 the influence of muscle mass could be less; in 2 not all fibers are recruited because the stimulation duration is too short to reach maximum force and in 3 there is time for reorganization during the interruption of the stimulus.
The increase in force during an isometric bladder contraction can be described by the time constant (. In striated muscle, ( is in the order of 3-100 ms (Joumaa and Leoty, 2002; Luff, 1981) and in urinary bladder ( is about 3 s (le Feber et al., 1997; van Koeveringe and van Mastrigt, 1991). We found that the redevelopment force immediately after shortening could be described by two time constants, one similar and one 3-5 times smaller than the isometric time constant which suggests that two different processes are involved.
Peiper et al. found that the initial force increase in rat tracheal muscle could adequately be described by a mono-exponential function (Peiper et al., 1984). After an imposed oscillation protocol, the redevelopment force was described by two time constants. The initial fast component presumably reflected rearrangement or reattachment of those cross-bridges previously detached by the quick length change whereas the slow component reflects the kinetics of the normal actin-myosin interaction. ADDIN EN.CITE Peiper198434065046940031-676840211984SepThe time course of changes in contraction kinetics during the tonic activation of the rat tracheal smooth muscle83-7Peiper, U.Vahl, C. F.Donker, E.Pflugers ArchActins/physiologyAnimalBiomechanicsElectric StimulationFemaleKineticsMale*Muscle ContractionMuscle TonusMuscle, Smooth/*physiologyMyosins/physiologyRabbitsRatsRats, Inbred StrainsTime FactorsTrachea/physiologyVibrationhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=6504694Peiper199433077678760008-421272111994NovSmooth muscle contraction kinetics at different calcium concentrations1338-44Institute of Physiology, University Hospital Eppendorf, University of Hamburg, Germany.Peiper, U.Dee, J.Can J Physiol PharmacolActins/physiologyAnimalBarium/pharmacologyCalcium/*physiologyCalcium Chloride/pharmacologyDown-Regulation/drug effectsElectric StimulationEthers, Cyclic/pharmacologyExtracellular Space/metabolismIn VitroIsometric Contraction/physiologyKineticsMuscle Contraction/drug effects/physiologyMuscle, Smooth, Vascular/drug effects/metabolism/*physiologyMyosins/physiologyOkadaic AcidOsmolar ConcentrationPhosphoprotein Phosphatase/antagonists & inhibitorsRatshttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=7767876Meiss199631087699900002-95132712 Pt 11996AugPotentiation of smooth muscle contractility after rapid changes in isotonic forceC511-23Department of Physiology, Indiana University School of Medicine, Indianapolis 46202, USA.Meiss, R. A.Am J PhysiolAnimalBiomechanicsElasticityFemaleIsometric Contraction*Isotonic ContractionLigamentsModels, Biological*Muscle ContractionMuscle, Smooth/*physiologyOvaryRabbitsTime Factorshttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=8769990(Meiss, 1996; Peiper et al., 1984; Peiper and Dee, 1994; Yamakawa et al., 1990). It is known that under certain circumstances in addition to normal bridges so called latch bridges are formed that maintain force at very low energy expense. Latch bridges are formed only after a considerable period of stimulation, f.i. after 20 s to 5 min in taenia coli in the steady state condition (Hellstrand, 1994). Since in our experiments the stimulation duration before shortening never exceeded 14 s, we assume that there were no latch bridges during the applied shortening.
During a contraction, the measurable intracellular calcium concentration decreases before the maximum force is reached (Arner et al., 1998). Presumably calcium is released into the cytoplasm and then taken up by the sarcoplasmatic reticulum (SR). In our experiments with a relatively short stimulation duration before shortening, calcium may not have been (completely) internalized in the SR and immediate availability could explain the better recovery compared to that measured with a longer stimulation duration. Leach et al. found in cardiac muscle that in the absence of the SR there is no deactivation. (Leach et al., 1999). Gunst has shown that with electrical stimulation the cytoplasmic calcium concentration after shortening is high and related to the length change (Gunst, 1989). The post vibration force recovery in rat portal vein was complete and the time constant decreased with an increase in calcium concentration when electrically stimulated (Peiper and Dee, 1994). Taking these findings into account, we suggest that in our data, (1 describes normal cycling, requiring extracellular calcium influx and/or release from intracellular stores whereas the smaller (2 is based on the immediate availability of cytoplasmic calcium. The stimulation interruption after shortening enables reuptake of most of the cytoplasmic calcium by the SR, restoring the starting conditions for a contraction; the fast component ((2) disappears. The finding of a single time constant after interruption, similar to the time constant at start and stop length, suggests that in this case redevelopment force is generated as during a normal isometric contraction.
In striated muscle, a short interruption of stimulation after active shortening (1-2 s) allows a full recovery of force, presumably because all cross-bridges detach and actin and myosin are able to form new bridges again ADDIN EN.CITE Morgan2000430Morgan, D.L.Whitehead, N.P.Wise, A.K.Gregory, J.E.Proske, U.2000Tension changes in the cat soleus muscle following slow stretch or shortening of the contracting muscleJ Physiol5223503 - 513de Ruiter1998140de Ruiter, CJde Haan, AJones, DASargeant, AJ1998Shortening-induced force depression in human adductor pollicis muscleJ Physiol5072583 - 591(Morgan et al., 2000; de Ruiter et al., 1998). In our measurements in smooth muscle, complete redevelopment of force required an interruption of 6 to 12 s.
Overall, we found that shortening induced deactivation increases with the shortening amplitude and shortening delay while it decreases with the interruption time after shortening.
We suggest that deactivation is caused by the disorganisation of filaments during shortening and by the subsequent relatively slow adaptation to the new length. Force development can be described by a single time constant but immediately after shortening, a second faster process becomes involved. We think that this process is caused by the immediate availability of cytoplasmic calcium. We intend to test this hypothesis in future experiments by changing the exctracellular calcium concentration (buffer) and/or by influencing the intracellular calcium concentration by blocking sarcoplasmatic reticulum receptors.
ACKNOWLEDGEMENT
The Technology Foundation STW, applied division of the Netherlands Organization for Scientific Research (NWO) and the technology program of the Ministry of Economic affairs supported this research.
REFERENCES
Arner, A., Malmqvist, U., Rigler, R., 1998. Calcium transients and the effect of a photolytically release calcium chelator during electrically induced contractions in rabbit rectococcygeus smooth muscle. Biophysical Journal 75, 1895-1903.
Asselt, E. van, Pel, J., van Mastrigt, R., 2002. Shortening induced deactivation in pig urinary smooth muscle. Journal of Muscle Research and Cell Motility 23, 37.
Colomo, F., Poggesi, C., Tesi, C., 1994. Force responses to rapid length changes in single intact cells from frog heart. Journal of Physiology 475, 347-350.
Corr, D.T., Herzog, W., 2005. Force recovery following activated shortening in whole skeletal muscle: transient and steady-state aspects of force depression. Journal of Applied Physiology 99, 252 260.
Feber, J. le, van Asselt, E., van Mastrigt, R., 1997. Neurophysiological modeling of voiding in rats: bladder pressure and postganglionic bladder nerve activity. American Journal of Physiology 272, R413-421.
Gunst, S., 1986. Effect of length history on contractile behavior of canine tracheal smooth muscle. American Journal of Physiology 250, C146-154.
Gunst, S., 1989. Effects of muscle length and load on intracellular Ca2+ in tracheal smooth muscle. American Journal of Physiology 256, C807-812.
Gunst, S., Meiss, R., Wu, M., Rowe, M., 1995. Mechanisms for the mechanical plasticity of tracheal smooth muscle. American Journal of Physiology 268, C1267-1276.
Gunst, S., Wu, M., 2001. Selected contribution: plasticity of airway smooth muscle stiffness and extensibility: role of length-adaptive mechanisms. Journal of Applied Physiology 90, 741-749.
Gunst, S., Wu, M., Smith, D., 1993. Contraction history modulates isotonic shortening velocity in smooth muscle. American Journal of Physiology 265, C467-476.
Hellstrand, P., 1994. Cross-bridge kinetics and shortening in smooth muscle. Canadian Journal of Physiology and Pharmacology 72, 1334-1337.
ADDIN EN.REFLIST Herzog, W., Leonard, T., 1997. Depression of cat soleus forces following isokinetic shortening. Journal of Biomechanics 30, 865-872.
Herzog, W., Leonard, T., Wu, J., 2000. The relationship between force depression following shortening and mechanical work in skeletal muscle. Journal of Biomechanics 33, 659-668.
Joumaa, W., Leoty, C., 2002. A comparative analysis of the effects of exercise training on contractile responses in fast- and slow twitch rat skeletal muscle. Journal of Comparative Physiology (B) 172, 329 -338.
Koeveringe, G. van, 1997. Dynamics of smooth muscle contraction. PhD.thesis, Rotterdam: Erasmus University, Rotterdam.
Koeveringe, G. van, van Mastrigt, R., 1991. Excitatory pathways in smooth muscle investigated by phase-plot analysis of isometric force development. American Journal of Physiology 261, R138-144.
Leach, J.K., Priola, D.V., Grimes, L.A., Skipper, B.J., 1999. Shortening deactivation of cardiac muscle: physiological mechanisms and clinical implications. Journal of Investigative Medicine 47, 369-377.
Leonard, T.R., Herzog, W., 2005. Does the speed of shortening affect steady-state force depression in cat soleus muscle ? Journal of Biomechanics 38, 2190 -2197
Luff, A., 1981. Dynamic properties of the inferior rectus, extensor digitorum longus, diaphragm and soleus muscles of the mouse. Journal of Physiology 313, 161-171.
Marechal, G., Plaghki, L., 1979. The deficit of the isometric tetanic tension redeveloped after a release of frog muscle at a constant velocity. Journal of General Physiology 73, 453-467.
Mehta, D., Wu, M., Gunst, S., 1996. Role of contractile protein activation in the length-dependent modulation of tracheal smooth muscle force. American Journal of Physiology 270, C243-252.
Meiss, R., 1993. Persistent mechanical effects of decreasing length during isometric contraction of ovarian ligament smooth muscle. Journal of Muscle Research and Cell Motility 14, 205-218.
Meiss, R., 1996. Potentiation of smooth muscle contractility after rapid changes in isotonic force. American Journal of Physiology 271, C511-523.
Minekus, J., van Mastrigt, R., 2001. Length dependence of the contractility of pig detrusor smooth muscle fibres. Urological Research 29, 126-133.
Morgan, D., Whitehead, N., Wise, A., Gregory, J., Proske, U., 2000. Tension changes in the cat soleus muscle following slow stretch or shortening of the contracting muscle. Journal of Physiology 522, 503-513.
Peiper, U., Dee, J., 1994. Smooth muscle contraction kinetics at different calcium
concentrations. Canadian Journal of Physiology and Pharmacology 72, 1338-1344.
Peiper, U., Vahl, C., Donker, E., 1984. The time course of changes in contraction kinetics during the tonic activation of the rat tracheal smooth muscle. Pflgers Archives 402, 83-87.
Pratusevich, V., Seow, C., Ford, L., 1995. Plasticity in canine airway smooth muscle. Journal of General Physiology 105, 73-94.
Ruiter, C. de, de Haan, A., Jones, D., Sargeant, A., 1998. Shortening-induced force depression in human adductor pollicis muscle. Journal of Physiology 507, 583-591.
Shen, X., Wu, M., Tepper, R., Gunst, S., 1997. Mechanisms for the mechanical response of airway smooth muscle to length oscillation. Journal of Applied Physiology 83, 731-738.
Suga, H., Yamakoshi, K., 1977. Effects of stroke volume and velocity of ejection on end-systolic pressure of canine left ventricle. End-systolic volume clamping. Circulation Research 40, 445-450.
Yamakawa, M., Harris, D.E., Fay, F.S., Warshaw, D.M., 1990. Mechanical transients of single toad stomach smooth muscle cells. Journal of General Physiology 95, 697 - 715.
LEGENDS
Figure 1
Example of a stoptest measurement with the stimulation turned off and on.
The passive force ( ) subtracted from the total force (-----) yielded the active force ( ). The active force increased to a maximum value (Fmax). After shortening the muscle bundle from start length (lstart) to stop length (lstop) with a pre-set velocity (vshort), the force decreased to a minimum (Fmin) and redeveloped to an isometric value (Fred). The hatched area is an estimate for the amount of work performed during shortening. After 5 min an isometric contraction at stop length was evoked with a maximum active force of Fiso. Shortening deactivation was defined as Fred/Fiso.
Figure 2
Examples of the three series of stoptest measurements. In series 1 the shortening amplitude (lshort) was varied (panel A). In series 2 the shortening delay (tdelay) was varied (panel B) and in series 3 the stimulus interruption time (tint) was varied (panel C). All panels show active muscle force, muscle length and stimulus against time. A scale for force and time is added. For details see Materials and Methods.
Figure 3
The redevelopment force immediately after shortening without interruption, was described by two separate processes: a slow ((1) contribution to force redevelopment with a large amplitude (F1) and a fast ((2) contribution with a small amplitude (F2)
Figure 1
Figure 2
Figure 3
Table I
Mean SD of deactivation (Fred/Fiso and Fred Fiso) and decrease in force during shortening (Fmax Fmin), n is the number of contractions.
Linear regression and the Spearmans correlation test. * correlation is significant at the 0.01 level (2-tailed)
Fred/FisoFred-Fiso (N*10-3)Fmax-Fmin(N*10-3)nlshort ((m): 1000.86 0.09-2.1 1.84.5 1.313 2000.82 0.08-2.3 1.38.1 2.413 3000.80 0.09-3.0 1.810.5 3.113 4000.78 0.07-3.1 1.711.7 3.517 7000.69 0.07-4.0 1.814.2 5.013 mean0.79 0.10-2.9 1.89.9 4.669Linear regression-2.6x10-4-3.2x10-31.5x10-2Correlation 0.54*0.370.67*tdelay(s) 140.70 0.10-3.2 0.87.6 2.210 110.76 0.10-2.5 0.68.3 2.67 80.83 0.08-1.9 0.57.5 2.37 50.86 0.06-1.7 0.65.4 1.77 30.91 0.04-1.2 0.63.0 1.87 20.92 0.03-1.0 0.5-0.08 0.67 mean0.82 0.11-2.0 1.05.5 3.545Linear regression-1.8x10-2-0.180.56Correlation 0.74*0.80*0.72*
tint (s): 00.81 0.05-2.8 0.713.0 1.3520.84 0.06-2.1 1.314.3 3.71530.79 0.05-4.0 3.216.5 7.4840.85 0.04-3.1 2.415.6 7.21060.88 0.09-1.8 1.313.8 3.913121.10 0.201.0 2.111.7 0.62221.12 0.151.2 1.411.7 1.12mean0.86 0.11-2.3 2.214.5 5.155Linear regression1.6x10-20.222.5x10-2Correlation 0.65*0.44*0.009
Table II
Analysis of the relationship between deactivation (Fred/Fiso and Fred Fiso) and decrease in force during shortening (Fmax Fmin), work (W), the relative amplitude of the slow force component (%F1) and the quotient of both force amplitudes (F2/F1) resulting from fitting a bi-exponential function to the redevelopment force after shortening.
Spearmans correlation test, * is significant at the 0.01 level (2-tailed)
Fmax Fmin (N)Work (Nm)%F1F2/F1nSeries 1Fred / Fiso- 0.51*- 0.57*0.05 0.1547Fred - Fiso- 0.76*- 0.71*0.04 0.0847Series 2Fred / Fiso- 0.56*- 0.39*0.10- 0.1143Fred - Fiso- 0.78*- 0.68* 0.40* - 0.40*43Series 3Fred / Fiso- 0.27 0.19--46Fred - Fiso- 0.75*- 0.22--46
Table III
Mono- and bi-exponential functions were used to describe the rate of force development at start length, immediately after shortening at stop length and at isometric stimulation, 5 min after the stop test at stop length (mean SD of (mono, (1 and (2)
Fit errors (mean qmono/bi SD) were calculated by dividing the sum of the absolute differences between measured and fitted force values by the sum of the measured force values. n is the number of measurements. The bi-exponential fit was considered valid only in the redevelopment contractions of series 1 and 2, in all other cases n was to small or (1 was equal to ( 2 .
* : ( 1 i s s i g n i f i c a n t l y d i f f e r e n t f r o m ( m o n o , * * : ( 2 i s s i g n i f i c a n t l y d i f f e r e n t f r o m ( 1 ( p <