Binary typing of Staphylococcus aureus strains through reversed hybridization using digoxigenin-universal linkage system-labeled bacterial genomic DNA.
A novel binary typing (BT) procedure, based on reversed hybridization of digoxigenin-universal linkage system-labeled bacterial DNA to strip-immobilized probes, is presented. Chromogenic detection of hybrids was performed. Staphylococcus aureus isolates (n = 20) were analyzed to establish the feasibility of BT. A technically simple and fast procedure has been developed for application in routine microbiology laboratories.
|Keywords||*Bacterial Typing Techniques, *Digoxigenin, Chromogenic Compounds, Comparative Study, DNA Probes/*genetics, DNA, Bacterial/analysis/*genetics, Electrophoresis, Gel, Pulsed-Field, Genome, Bacterial, Humans, Nucleic Acid Hybridization/methods, Random Amplified Polymorphic DNA Technique, Research Support, Non-U.S. Gov't, Staphylococcal Infections/*microbiology, Staphylococcus aureus/*classification/genetics|
|Persistent URL||dx.doi.org/10.1128/JCM.39.1.328-331.2001, hdl.handle.net/1765/12914|
van Leeuwen, W., Libregts, C., Schalk, M., Veuskens, J., Verbrugh, H.A., & van Belkum, A.F.. (2001). Binary typing of Staphylococcus aureus strains through reversed hybridization using digoxigenin-universal linkage system-labeled bacterial genomic DNA.. Journal of Clinical Microbiology, 39(1), 328–331. doi:10.1128/JCM.39.1.328-331.2001