T cell-independent development and induction of somatic hypermutation in human IgM+IgD+CD27+ B cells
IgM+IgD+CD27+ B cells from peripheral blood have been described as circulating marginal zone B cells. It is still unknown when and where these cells develop. These IgM+IgD +CD27+ B cells exhibit somatic hypermutations (SHMs) in their B cell receptors, but the exact nature of the signals leading to induction of these SHMs remains elusive. Here, we show that IgM+IgD +CD27+ B cells carrying SHMs are observed during human fetal development. To examine the role of T cells in human IgM +IgD+CD27+ cell development we used an in vivo model in which Rag2-/-γc-/- mice were repopulated with human hematopoietic stem cells. Using Rag2 -/-γc-/- mice on a Nude background, we demonstrated that development and induction of SHMs of human IgM +IgD+CD27+ B cells can occur in a T cell - independent manner.
|Keywords||Animals, Antigens, B lymphocyte, B-Lymphocytes, CD27, CD27 antigen, Fetal Blood, Hematopoietic Stem Cells, Humans, Immunoglobulin D, Immunoglobulin M, Inbred BALB C, Knockout, Mice, Mutation, Nude, Signal Transduction, T lymphocyte, T-Lymphocytes, article, controlled study, fetus development, hematopoietic stem cell, human, human cell, immunoglobulin D, immunoglobulin M, in vivo study, mouse, nonhuman, priority journal, somatic hypermutation|
|Persistent URL||dx.doi.org/10.1084/jem.20070447, hdl.handle.net/1765/15212|
Scheeren, F.A., Nagasawa, M., Weijer, K., Cupedo, T., Kirberg, J., Legrand, N., & Spits, H.. (2008). T cell-independent development and induction of somatic hypermutation in human IgM+IgD+CD27+ B cells. The Journal of Experimental Medicine, 205(9), 2033–2042. doi:10.1084/jem.20070447