The human β-globin gene contains multiple regulatory regions: identification of one promoter and two downstream enhancers.
We have introduced into murine erythroleukaemia (MEL) cells several series of deletion mutants of hybrid genes between the human beta-globin gene and the murine H-2Kb gene. S1 nuclease and transcriptional run-off analysis showed that the human beta-globin gene contains at least three globin specific transcriptional control elements. One is a promoter element and is located 160 bp upstream from the transcription initiation site; it increases the efficiency of the promoter after MEL cells are induced to differentiate. The other two elements are tissue-specific transcriptional enhancers and are located downstream from the transcription initiation site, the first in the structural beta-globin gene and the second in the 3' flanking sequences.
|Keywords||*Enhancer Elements (Genetics), *Genes, Regulator, *Genes, Structural, *Promoter Regions (Genetics), 9004-22-2 (Globins), Amino Acid Sequence, Animals, Base Sequence, Cell Line, Chromosome Deletion, DNA Restriction Enzymes, EC 3.1.21 (DNA Restriction Enzymes), Globins/*genetics, Hela Cells/metabolism, Human, Leukemia, Erythroblastic, Acute, Molecular Sequence Data, Support, Non-U.S. Gov't, Transfection|
Antoniou, M., de Boer, E., Habets, G., & Grosveld, F.G.. (1988). The human β-globin gene contains multiple regulatory regions: identification of one promoter and two downstream enhancers.. EMBO Journal, 7, 377–384. Retrieved from http://hdl.handle.net/1765/2426