The human β-globin gene 3' enhancer contains multiple binding sites for an erythroid specific protein.
We have shown that the minimal enhancer fragment present in the 3'-flanking region of the human beta-globin gene contains four regions that bind nuclear proteins in vitro. By using gel mobility shift and DNase I footprinting assays, we were able to show that each of these regions binds an erythroid-cell-specific nuclear factor which we name NF-E1. This factor is present in erythroid cells at different developmental stages of globin gene expression. The recognition sequence of this protein (A/C Py T/A ATC A/T Py) is also present in the intragenic enhancer and the promoter of the beta-globin gene as well as in the promoter of other erythroid-cell specific genes. In addition to NF-E1, each of the four binding regions interacts with at least one other protein factor.
|Keywords||*Enhancer Elements (Genetics), *Erythropoiesis, *Transcription Factors, 0 (Beta-Globulins), 0 (DNA-Binding Proteins), 0 (Transcription Factors), 125267-48-3 (erythroid-specific DNA-binding factor), Base Sequence, Beta-Globulins/*genetics, Binding Sites, DNA-Binding Proteins/*metabolism, Deoxyribonuclease I/metabolism, EC 220.127.116.11 (Deoxyribonuclease I), Human, Molecular Sequence Data, Nucleotide Mapping, Organ Specificity, Support, Non-U.S. Gov't|
Wall, L., de Boer, E., & Grosveld, F.G.. (1988). The human β-globin gene 3' enhancer contains multiple binding sites for an erythroid specific protein.. Genes & Development, 2, 1089–1100. Retrieved from http://hdl.handle.net/1765/2431