Molecular characterization of the human excision repair gene ERCC-1: cDNA cloning and aminoacid homology with the yeast DNA repair gene RAD10.
The human excision repair gene ERCC-7 was cloned after DNA mediated gene transfer to the CHO mutant 43-38, which is sensitive to ultraviolet light and mitomycin-C. We describe the cloning and sequence analysis of the ERCC-7 cDNA and partial characterization of the gene. ERCC.1 has a size of 15 kb and is located on human chromosome 19. The ERCC.1 precursor RNA is subject to alternative splicing of an internal 72 bp coding exon. Only the cDNA of the larger 1.1 kb transcript, encoding a protein of 297 amino acids, was able to confer resistance to ultraviolet light and mitomycin-C on 43-38 cells. Significant amino acid sequence homology was found between the ERCC.7 gene product and the yeast excision repair protein RADIO. The most homologous region displayed structural homology with DNA binding domains of various polypeptides.
|Keywords||*Aspartate Carbamoyltransferase, *Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing), *Chromosome Deletion, *Cloning, Molecular, *DNA Repair, *Dihydroorotase, *Genes, *Genes, Fungal, *Multienzyme Complexes, 0 (CAD trifunctional enzyme), 0 (DNA-Binding Proteins), 0 (Multienzyme Complexes), 0 (Proteins), 0 (RNA, Messenger), 24937-83-5 (Poly A), 63231-63-0 (rna), 9007-49-2 (dna), Amino Acid Sequence, Animals, Base Sequence, Chromosome Mapping, Cricetulus, DNA-Binding Proteins/analysis, DNA/*analysis, EC 184.108.40.206 (Aspartate Carbamoyltransferase), EC 220.127.116.11 (Dihydroorotase), EC 18.104.22.168 (Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)), Evolution, Genetic Complementation Test, Hamsters, Hela Cells, Human, Poly A/metabolism, Proteins/analysis, RNA, Messenger, RNA/metabolism, Saccharomyces cerevisiae/*genetics, Support, Non-U.S. Gov't|
|Persistent URL||dx.doi.org/10.1016/0092-8674(86)90014-0, hdl.handle.net/1765/2990|
van Duin, M., de Wit, J., Odijk, H., Westerveld, A., Yasui, A., Koken, M.H.M., … Bootsma, D.. (1986). Molecular characterization of the human excision repair gene ERCC-1: cDNA cloning and aminoacid homology with the yeast DNA repair gene RAD10.. Cell, 44(6), 913–923. doi:10.1016/0092-8674(86)90014-0