Genomic characterization of the human DNA excision repair gene ERCC-1.
In this report the genomic characterization of the human excision repair gene ERCC-1 is presented. The gene consists of 10 exons spread over approximately 15 kb. By means of a transfection assay the ERCC-1 promoter was confined to a region of + 170 bp upstream of the transcriptional start site. Classical promoter elements like CAAT, TATA and GC-boxes are absent from this region. Furthermore, ERCC-1 transcription is not UV-inducible. A possible explanation is provided for the previously reported alternative splicing of exon VIII. Analysis of ERCC-1 cDNA clones revealed the occurrence of differential polyadenylation which gives ERCC-1 transcripts of 3.4 and 3.8 kb in addition to the major 1.1 kb mRNA. Apparent evolutionary conservation of differential polyadenylation of ERCC-1 transcripts suggests a possible role for this mode of RNA processing in the ERCC-1 repair function.
|Keywords||*DNA Repair, *Genes, 0 (Plasmids), 9007-49-2 (dna), Animals, Base Sequence, Cell Line, DNA/isolation & purification, Hela Cells/metabolism, Human, Molecular Sequence Data, Plasmids, Promoter Regions (Genetics), Support, Non-U.S. Gov't, Transfection, Ultraviolet RaysISSUED 1987|
van Duin, M., Koken, M.H.M., van den Tol, J., ten Dijke, P., Westerveld, A., Bootsma, D., & Hoeijmakers, J.H.J.. (1987). Genomic characterization of the human DNA excision repair gene ERCC-1.. Nucleic Acids Research, 15, 9195–9213. Retrieved from http://hdl.handle.net/1765/2993