Accelerated viraemia in cats vaccinated with fixed autologous FIV-infected cells. Abstract 2nd International Feline Immunology Workshop, 31 July-3 August 1997, Davis, USA.
We have vaccinated cats with fixed autologous FIV infected PBMC to determine whether autologous presentation of antigen is capable of inducing a protective immune response against homologous challenge. To this end autologous PBMC were infected with a FIV molecular clone (19k1). When infection was established, cells were inactivated by dialysis against paraformaldehyde. Upon vaccination, cats developed a virus specific immune response as measured by ELISA against the Gag protein of FIV. No antibodies against the envelope protein were detected with a peptide ELISA. Virus neutralizing antibodies however could be detected with a neutralization assay based on infection of CrFK cells, but not in an assay based on infection of primary T-cells. Although vaccination led to the induction of these virus-specific immune responses, vaccinated cats were not protected against homologous challenge but showed an accelerated viraemia upon infection. This was shown both by PCR and cell-associated viral load. The possible mechanisms underlying this observation are discussed in this paper.
|Keywords||Animals, Antibodies, Viral/analysis, Blood Component Transfusion, Blood Transfusion, Autologous, Cats, DNA Primers/chemistry, DNA, Viral/analysis, Feline Acquired Immunodeficiency Syndrome/immunology/*prevention & control, Immunodeficiency Virus, Feline/genetics/*immunology, Polymerase Chain Reaction/veterinary, T-Lymphocytes/immunology/*virology, Vaccination/*veterinary, Viral Load, Viral Vaccines/*administration & dosage, Viremia/*etiology/immunology|
|Persistent URL||dx.doi.org/10.1016/S0165-2427(98)00166-4, hdl.handle.net/1765/3659|
Karlas, J.A., Siebelink, C.H.J., van Peer, M.A., Huisman, W., Rimmelzwaan, G.F., & Osterhaus, A.D.M.E.. (1998). Accelerated viraemia in cats vaccinated with fixed autologous FIV-infected cells. Abstract 2nd International Feline Immunology Workshop, 31 July-3 August 1997, Davis, USA.. Veterinary Immunology and Immunopathology, 65(2-4), 353–365. doi:10.1016/S0165-2427(98)00166-4