Phosphorylation of cardiac myofilament proteins represents one of the main post-translational mechanisms that regulate cardiac pump function. Human studies are often limited by the amount of available tissue as biopsies taken during cardiac catheterization weigh only 1 mg (dry weight). Similarly, investigation of time- (or dose-) dependent changes in protein phosphorylation in animal studies is often hampered by tissue availability. The present study describes quantitative analysis of phosphorylation status of multiple myofilament proteins by 2-DE and Pro-Q® Diamond stained gradient gels using minor amounts (-0.5 mg dry weight) of human and pig cardiac tissue.

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Keywords 2-DE, Cardiac tissue, Pro-Q® Diamond stain, Protein phosphorylation, TCA
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Zaremba, R, Merkus, D, Hamdani, N, Lamers, J.M.J, Paulus, W.J, dos Remedios, C, … van der Velden, J. (2007). Quantitative analysis of myofilament protein phosphorylation in small cardiac biopsies. Proteomics - Clinical Applications, 1(10), 1285–1290. doi:10.1002/prca.200600891