In vivo antibody response and in vitro CTL activation induced by selected measles vaccine candidates, prepared with purified Quil A components.
Semipurified Quil A and purified Quil A were used to prepare well-characterized subunit vaccine candidates against measles. Variation in the relative amounts of the measles virus (MV) fusion (F) protein, Quil A-components and lipids did not influence induction of antibody responses in mice, but had a pronounced effect on the capacity to induce cytotoxic T cell (CTL) activity of a CD8(+) MV F-protein specific human T cell clone in vitro. A characteristic MV iscom preparation based on the combined use of HPLC-purified Quil A-components QA-3 and QA-22 (QA-3/22) efficiently induced CTL activity in vitro. Comparable results were obtained by mixing beta-propiolactone inactivated MV with iscom-matrix QA-3/22 or free QA-22. On the basis of the data presented it was concluded that these three preparations are interesting MV vaccine candidates for further evaluation in pre-clinical experiments in a primate model.
|Keywords||*Lymphocyte Activation, Adjuvants, Immunologic/*chemistry/isolation & purification, Animals, Antibodies, Viral/*biosynthesis, Cells, Cultured, Cercopithecus aethiops, Chromatography, High Pressure Liquid, Cytotoxicity, Immunologic, Hemagglutinins, Viral/*immunology, Humans, ISCOMs/chemistry/*immunology, Measles Vaccine/chemistry/*immunology, Measles virus/drug effects/*immunology, Mice, Mice, Inbred BALB C, Microscopy, Electron, Propiolactone/pharmacology, Saponins/chemistry/*immunology, T-Lymphocytes, Cytotoxic/*immunology, Vaccination, Vaccines, Attenuated/immunology, Vero Cells, Viral Fusion Proteins/*immunology|
|Persistent URL||dx.doi.org/10.1016/S0264-410X(00)00026-8, hdl.handle.net/1765/3722|
Stittelaar, K.J., Boes, J., Spiekstra, A., de Vries, P., Roholl, P.J.M., Dalsgaard, K., … Mulder, P.G.H.. (2000). In vivo antibody response and in vitro CTL activation induced by selected measles vaccine candidates, prepared with purified Quil A components.. Vaccine, 18(23), 2482–2493. doi:10.1016/S0264-410X(00)00026-8