Characterization of Fxr1 in Danio rerio; a simple vertebrate model to study costamere development
The X-linked FMR1 gene, which is involved in the fragile X syndrome, forms a small gene family with its two autosomal homologs, FXR1 and FXR2. Mouse models for the FXR genes have been generated and proved to be valuable in elucidating the function of these genes, particularly in adult mice. Unfortunately, Fxr1 knockout mice die shortly after birth, necessitating an animal model that allows the study of the role of Fxr1p, the gene product of Fxr1, in early embryonic development. For gene function studies during early embryonic development the use of zebrafish as a model organism is highly advantageous. In this paper the suitability of the zebrafish as a model organism to study Fxr1p function during early development is explored. As a first step, we present here the initial characterization of Fxr1p in zebrafish. Fxr1p is present in all the cells from zebrafish embryos from the 2/4-cell stage onward; however, during late development a more tissue-specific distribution is found, with the highest expression in developing muscle. In adult zebrafish, Fxr1p is localized at the myoseptum and in costamere-like granules in skeletal muscle. In the testis, Fxr1p is localized in immature spermatogenic cells and in brain tissue Fxr1p displays a predominantly nuclear staining in neurons throughout the brain. Finally, the different tissue-specific isoforms of Fxr1p are characterized. Since the functional domains and the expression pattern of Fxr1p in zebrafish are comparable to those in higher vertebrates such as mouse and human, we conclude that the zebrafish is a highly suitable model for functional studies of Fxr1p.
|Keywords||*Gene Expression Profiling, *Models, Animal, Amino Acid Sequence, Animals, Base Sequence, Blotting, Western, Comparative Study, Cryoultramicrotomy, DNA Primers, Immunohistochemistry, Molecular Sequence Data, RNA-Binding Proteins/metabolism, Research Support, Non-U.S. Gov't, Sequence Alignment, Sequence Analysis, DNA, Zebrafish/genetics/*metabolism|
Engels, B., van 't Padje, S., Blonden, L., Severijnen, L.A., Oostra, B.A., & Willemsen, R.. (2004). Characterization of Fxr1 in Danio rerio; a simple vertebrate model to study costamere development. The Journal of Experimental Biology. Retrieved from http://hdl.handle.net/1765/8404