Natural infection with herpes simplex virus type 1 (HSV-1) induces humoral and T cell responses to the HSV-1 glycoprotein H:L complex
The glycoproteins of herpes simplex virus type 1 (HSV-1) are important targets for the immune system in the control of HSV-1 infections. The humoral and T cell responses to the glycoprotein (g)H(t(His)):gL complex of HSV-1 were studied in seven HSV-1-seropositive and three HSV-1-seronegative healthy adults. In addition, responses to HSV-1 gD(t) were determined. As antigens, purified soluble recombinant forms of the gH(t(His)):gL complex produced by insect cells and of gD(t) produced by yeast cells were used. In contrast to seronegative donors, sera of all seropositive donors contained gH(t(His)): gL-specific IgG. Using peripheral blood (PB) T cells, gH(t(His)):gL-specific proliferative T cell responses were detected in all seropositive donors. Culture supernatants of PB T cells stimulated with recombinant gH(t(His)):gL contained high levels of interferon-gamma and no detectable interleukin-4, indicating their Th1 phenotype. These results show that naturally acquired HSV-1 infection induces gH:gL-specific humoral and T cell responses.
|Keywords||Animals, Antibodies, Viral/*blood, Cercopithecus aethiops, Herpesvirus 1, Human/*immunology, Humans, Interferon Type II/biosynthesis, Interleukin-4/biosynthesis, Lymphocyte Activation, T-Lymphocytes/*immunology, Vero Cells, Viral Envelope Proteins/*immunology|
Westra, D.F., van Kooij, A., Welling, G.W., Scheffer, A.J., The, T.H., Welling-Wester, S., … Verjans, G.M.G.M.. (2000). Natural infection with herpes simplex virus type 1 (HSV-1) induces humoral and T cell responses to the HSV-1 glycoprotein H:L complex. Journal of General Virology. Retrieved from http://hdl.handle.net/1765/8472