Enzymatic method for inactivation of aminoglycosides during measurement of postantibiotic effect
To determine the postantibiotic effect of aminoglycosides, two methods are currently being used to remove the test drug: repeated washing and dilution. An enzymatic inactivation method of removing gentamicin and tobramycin was developed and compared with the dilution method. This enzymatic method provides a rapid and simple alternative method of removing aminoglycosides which results in reliable postantibiotic-effect values.
|Keywords||Acetyltransferases/*pharmacology, Anti-Bacterial Agents/*metabolism, Bacteria/drug effects/*metabolism, Comparative Study, Gentamicins/metabolism, Microbial Sensitivity Tests/*methods, Tobramycin/metabolism|
den Hollander, J.G., Mouton, J.W., Bakker-Woudenberg, I.A.J.M., Vleggaar, F.P., van Goor, M.P.J., & Verbrugh, H.A.. (1996). Enzymatic method for inactivation of aminoglycosides during measurement of postantibiotic effect. Antimicrobial Agents and Chemotherapy. Retrieved from http://hdl.handle.net/1765/8625