We have compared the efficacies of three general primer pairs for the detection of human papillomavirus (HPV) DNA in formaldehyde-fixed paraffin-embedded carcinomas. The use of these primer pairs leads to underestimates of the HPV prevalence (GP5/6, 61.1%; CPI/IIG, 57.4%; MY09/11, 46.9%; combined, 72.8%). The efficacy of each primer pair seemed to be inversely correlated to the length of the amplimer produced. By using newly developed type-specific primer pairs (amplimer length, approximately 100 bp), an increase in HPV DNA detection (87.6%) was found.

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hdl.handle.net/1765/8635
Journal of Clinical Microbiology
Erasmus MC: University Medical Center Rotterdam

Baay, M., Quint, W., Koudstaal, J., Hollema, H., Duk, J. M., Burger, M. P. M., … Herbrink, P. (1996). Comprehensive study of several general and type-specific primer pairs for detection of human papillomavirus DNA by PCR in paraffin-embedded cervical carcinomas. Journal of Clinical Microbiology. Retrieved from http://hdl.handle.net/1765/8635