Comprehensive study of several general and type-specific primer pairs for detection of human papillomavirus DNA by PCR in paraffin-embedded cervical carcinomas
We have compared the efficacies of three general primer pairs for the detection of human papillomavirus (HPV) DNA in formaldehyde-fixed paraffin-embedded carcinomas. The use of these primer pairs leads to underestimates of the HPV prevalence (GP5/6, 61.1%; CPI/IIG, 57.4%; MY09/11, 46.9%; combined, 72.8%). The efficacy of each primer pair seemed to be inversely correlated to the length of the amplimer produced. By using newly developed type-specific primer pairs (amplimer length, approximately 100 bp), an increase in HPV DNA detection (87.6%) was found.
|Keywords||*DNA Primers, *Polymerase Chain Reaction, Base Sequence, DNA, Viral/*analysis, Female, Humans, Molecular Sequence Data, Papillomavirus, Human/*genetics, Research Support, Non-U.S. Gov't, Uterine Cervical Neoplasms/*virology|
Baay, M.F.D., Quint, W.G.V., Koudstaal, J., Hollema, H., Duk, J.M., Burger, M.P.M., … Herbrink, P.. (1996). Comprehensive study of several general and type-specific primer pairs for detection of human papillomavirus DNA by PCR in paraffin-embedded cervical carcinomas. Journal of Clinical Microbiology. Retrieved from http://hdl.handle.net/1765/8635