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patient virus herpe strain infection simplex cornea sequence keratitis region herpetic -17 t cells il -17 study cytokine disease sample group chemokine secretion stromal result herpetic stromal keratitis herpes simplex virus effect genome number hypervariable regions culture amplicon sequential assay amplification superinfection expression chapter analysis factor transmission table comea fibroblast hsv infections herpetic keratitis pcr amplification method replication comeal analyses medium reactivation hsk patients development model activation difference probe protein induction response transplantation production ophthalmol pkp iek tissue tnf-a mouse control murine stimulation virol process ifn-y lesion length neutrophils rt-pcr epithelial hypervariable