Koolen, M.J.M.

virus mutant protein ts mutants virus-specific mouse van der zeijst mhv-a temperature mouse hepatitis virus hepatitis synthesis spaan plaque l cells wild-type strain rottier mhv-a 59 culture ter meulen wild-type virus virus-specific rnas infection balb /c mice leibowitz horzinek stock property coronaviruse intracellular study zeijst titer medium system characterization meulen 0.1 ml pbs lesion tissue 9 hr p.i virus stocks isolation nucleocapsid protein 0.001 0.003 koolen coronaviru coronavirus multiplication strategy mouse l cells experiment ts -209-infected cells 16- mm wells siddell virus clones murine wild-type mhv-a 59 rna synthesis neuropathogenic properties analysis species 10- manaker leakines actinomycin temperature-sensitive mutants plaque titers nucleocapsid structure virus growth clone plating control animals control protein synthesis ts -43 intracerebrally virus-specific proteins strategy