Boomaars, W.E.

cgk ii cgk i iec-cf 7 cells 125 i efflux protein membrane iec-cf n terminus chimer cgk chimer channel activation cftr cl channels myristoylation-deficient cgk ii terminus efflux cgk mutants kinase vaandrager activity substrate expression membrane binding properties localization phosphorylation mutant infection jonge lohmann cftr activation domain vector particle biochemistry fraction study cgmp-dependent protein kinase level peptide adenoviral binding myristoylation subcellular localization adenoviral vectors 0.1 n-terminal walter subcellular analog jarchau cgk ii activation cgk ii substrates 0.1 m anp 0.5 m nacl science immunofluorescence confocal microscopy addition plasma membrane 32 p incorporation cgk i mutants cgk ii-expressing cells membrane-permeable cgmp analogs cftr phosphorylation sequence cftr cl channel type i cgk cgk i chimer cytosolic fibrosis phosphorylate property regulator antibody confocal increase university absence immunoblotting effect transmembrane