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Putte, P. van de
(Pieter van de Putte)
vector clone plasmid library transfection selection system pecvh hprt cdna cell lines hatr clones expression cdna library sequence figure efficiency result acids research isolation interest number episomal 1150 bp pecvh 3-2 experiment research cell line transfected frequency transfection frequency transfection efficiency episome cdna inserts insert pecvh 2-2 hygromycin phenotype polyadenylation procedure fraction hypoxanthine model system dna uptake hypoxanthine phosphoribosyltransferase correction selection frequency ecori site 2-2 12roh cdna libraries sucrose gradient centrifugation k 562 cells ecori episomal vector system calf serum hprt mrna phenotypic correction xp 12roh cells alternative polyadenylation lesch-nyhan hoeijmaker month 3-2 subcloning region phenotypic medium bootsma westerveld culture generation ebv-derived microinjection transfectant pecv 25 xnmt-pcd orientation preparation transfectability population
2 Most Recent Publications
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RAD26, the functional S. cerevisiae homolog of the Cockayne syndrome B gene ERCC6.
(Article)
Gool, A.J. van Verhage, R. Swagemakers, S.M.A. Putte, P. van de Brouwer, J. Troelstra, C. Bootsma, D. Hoeijmakers, J.H.J. |
1994-01-01
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Efficient cDNA cloning by direct phenotypic correction of a mutant human cell line (HPRT-) using an Epstein-Barr virus derived cDNA expression vector.
(Article)
Belt, P.B.G.M. Jongmans, W. Wit, J. de Hoeijmakers, J.H.J. Backendorf, C.M.P. Putte, P. van de |
1991-01-01
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