Schouls, L.M.

pallidum antigen clone antibody protein treponemal aureus plasmid treponemal antigens strain lysate coli clones phenotype serum rabbit staphylococcus aureus cosmid staphylococcus hybridoma treponema fragment cluster number study coli k -12 syphilitic microbiol phagedeni syphilitic patients expression patient treponemal dna clone r 46 fragment length polymorphism pallidum cells pallidum dna treponema pallidum syphili sequence analysis anti-t -12 typing component immun method vector clonal complex tp 3a tween buffer genome polymorphism buffer deletion >30 polypeptide 44- pallidum antigen protein antigens syphilitic serum aflp data culture ug /ml antiserum treponema pallidum antigens 44- kd antigen density gradient-purified t tandem ht-aflp elisa production tween variability 800 clones type assignments antigenic 0.1 ml plasmid dna weight