Souren, R.M.

transporter transport protein sialic lysosomal membrane sialic acid transporter l-lactate neu 5ac activity column purification substrate buffer transport activity 1 min assay presence experiment proteoliposome monocarboxylate eluate proton gradient amersham pharmacia lactate carrier uptake fraction table 20 mm nahepes monosaccharide inhibition 40 mm mes preparation proton lysosomal membrane vesicles biochem disease erasmus mc table ii concentration hydroxyapatite study vesicle trans-stimulation gradient 50- l aliquot compound mct 1 buffer b q column sample inhibitor lectin reconstitution triton acidic lentil material sds-page glucuronic acid monocarboxylate transporters amersham liver aliquot acidic monosaccharides incubation system sialic acid l-lactate transport anion reconstitution assay pharmacia centricon 10 device storage cis-inhibition 100 mm kcl analysis pyruvate december