Bolk, M.W.

hiv -2 reaction substrate protein integration cleavage figure cleavage reaction dna ends binding sequence oligonucleotide aspecific product pmole double-stranded momlv aspecific dna hiv dna ends assay minute acids research competitor concentration shift 5 substrate result buffer condition strand virol sample target target dna dna binding dna substrates preparation mobility shift assay specificity mobility amount gel exclusion chromatography position 0.05 pmoles 3 pmoles 15 minutes figure 4 reaction mixtures double-stranded dna integration reactions hiv-l lanes 5 lanes 7 5 end 5 ends research competition lanes 9 activity polyacrylamide mixture 28- competitor dna denaturing room temperature analysis oligonucleotide substrates virus substrate concentration tris.hcl sequence specificity volume cleavage reactions nucleotide lanes 3 expression vol /vol glycerol im nacl mobility shift assays efficiency