Donk, H.J.M. van de

pallidum antigen clone antibody protein treponemal plasmid treponemal antigens lysate coli clones phenotype serum rabbit cosmid hybridoma treponema coli k -12 syphilitic phagedeni syphilitic patients expression patient treponemal dna clone r 46 pallidum cells pallidum dna treponema pallidum syphili fragment anti-t -12 component immun vector tp 3a tween buffer buffer deletion >30 polypeptide 44- strain pallidum antigen protein antigens syphilitic serum culture ug /ml antiserum treponema pallidum antigens 44- kd antigen density gradient-purified t number elisa production tween 800 clones study antigenic 0.1 ml plasmid dna weight gene bank fusion filament control embden stage method derivative nitrocellulose filters coli lysates sodium dodecyl sulfate-polyacrylamide 30 min bacteria pallidum immobilization test coli k -12. treponema pallidum dna 81/41 3-22 1982. van eijk