Broos, H.

antibody virus infection elisa immunodeficiency virus protein immunodeficiency serum immunodeficiency virus infection plate assay fiv infection antigen development fiv-specific e l i serum samples 153-165 sample siebelink protein-specific room temperature gag-specific antibodies pedersen rimmelzwaan culture volume detection env-specific antibodies response rimmelzwaanet al 10 min serum antibodies 16 hours culture supernatants crfk cells m t v fiv gag protein incubation virol weeks post infection env-specific c t b strain biotin hiv infection system ctb-elisa dutch microbiology spf cats fiv antigen petaluma strain osterhau adam 19 buffer 0.1 preparation a.d.m.e mtv-elisa result dilution gag-specific yamamoto supernatant phase mouse ascitic fluids fiv-specific mabs hammar 50/ xl volumes rec.env-elisa control antigen netherland temperature fiv-specific antibodies dutch cats 1991. fiv-specific serum antibodies egberink enzyme-linked immunosorbent assay