Janssens, R.J.

recombination plasmid clone vector e.coli sequence pac 148 frequency selection method modification integration ncoi site acids research pd 3lox plasmid pd 3lox type i region globin locus fragment globin reca gene rpsl gene homology recombinase e.coli dh 10b locus counter pac 100 pac clones mutation replication allele result e.coli reca gene genome figure loxp sequence 1.3 kb band pac 148 clones pac clone point mutations table counter selection pac 148 lox length loxp oligo recombination vector g-globin gene recombinases rece 1.0 kb bands globin pac clones research type ii co-integrants antibiotic natl acad l-agar plates mapping concentration 1.3 1.5 excision introduction procedure genomic rearrangement plate resistance co-integrant homology regions pdmcp 3f plasmid pbad-et tetr gene oligo deletion protein naei site pbad-et recombination plasmid pac vectors