Yasuda, M.

mper 2 protein mper 2 protein mcry proteins nucleus mcry 1 localization ubiquitylation presence clock figure degradation antibody export mper 2 proteins cos 7 cells accumulation mper 2. sequence subcellular circadian cytoplasmic absence domain ubiquitylated mper 2 subcellular localization cytoplasm nes sequences nes 1 region proteasome analysis mechanism ubiquitylated ubiquitin clock proteins system cytoplasmic localization transiently shearman 1/mcry 2±gfp residue level blot analysis crm 1/exportin nes domains distribution c-terminal region expression transfected hcry 2 export system nuclei mper 2±gfp proteins lysate mper 1 representative examples pattern amount experiment signal c-terminal mper 2±gfp co-expression mcry 1/mcry mice flag-tagged ubiquitin inhibitor mg 132 nes 3 protein synthesis mper 2 fig mcry 1 protein subcellular distribution patterns study gfp ¯ uorescence subcellular distribution pattern cell lysates mper 2 mrna immunoblot analysis