Assfalg-Machleidt, I.

calpain protein caspase apoptosi cleavage ionomycin bcl -2 proteins bcl -2 cytochrome c release bcl-xl 2 m ionomycin cytochrome ac 27p activation apoptotic family addition activity calcium cytochrome c pathway death mitochondria release ionomycin-induced concentration substrate result preincubation apoptotic pathway increase cell death cleavage sites calpain activation ionomycin-activated propidium iodide uptake binding inhibitor intracellular decrease ionomycin-activated calpain system ionomycin treatment treatment member process ionomycin-induced cell death study calpain-cleaved bcl -2 calpain-truncated bcl -2 mitochondrial contrast bcl -2 family calpain-cleaved enzyme bh 3 domains m-calpain 30 min subunit sample fluorescence munchen protease antibody ionomycin addition domain ionomycin-induced apoptosis actin cytosolic procedure z-devd-fmk ac-devd-amc propidium calpain-truncated membrane iodide chirurgische klinik innenstadt intracellular calcium concentration salvesen product