Zahler, S.

calpain subunit variant localization membrane activity calpain subunits domain 2 m ionomycin ionomycin 80 k subunit protein interaction image result antibody addition calpain activity fluorescence k-eyfp concentration intracellular subcellular subcellular localization nucleus study calpastatin condition activation overexpressed cos 7 cos 7 cells chimera n-terminal biochem fraction panel di-iib n terminus cytoplasmic 80 k-eyfp m-calpain calcium helice n-terminal helices overexpressing autolysi residue cell nucleus dissociation k-ecfp suzuki microscopy structure 30 k-eyfp 30 min cytoplasm 2 concentration expression transfected ecfp-di-iib cytoplasmic fraction di-iii calpain subunits fig 30 k subunit binding terminus 5- min intervals ecfp-diii enzyme chimeric analysis intracellular localization 2 concentrations plasma membrane table -80336 munchen ionomycin addition calpain variants transfected cells