Cziepluch, C.

protein hsc 70 bag -6 chromosome prometaphase 2 00 6 antibody misaligned chromosomes interaction analysis mitotic mitosi spindle poles population sirna hsp 70 control depletion spindle mitotic cells mislocalized chromosomes hsp 70 proteins misaligned hela cells extract metaphase plate result bag -6/bat flag-hsgt study arrest hsgt depletion cell division -6/bat microscopy death post-transfection binding fraction metaphase –25 hsgt-depleted domain hsgt-specific sirnas co-immunoprecipitation experiments partner function presence caspase -3 level plate point complex anaphase cell death division time points post-transfection time-lapse video microscopy contrast mitotic arrest mislocalized hsgt-specific time-lapse caspase video fluorescence hsgt-depleted cells cycle tubulin interphase control sirna tpr domain sgt 1 hsgt-specific sirna immunoblotting maldi-tof flag-tagged 49 h post-transfection delay polypeptide