Ruth, P

cgk ii autophosphorylation peptide activity ser 126 phosphorylation phosphorylated presence residue autophosphorylated sf 9 cells protein kinase 30 min autophosphorylation sites ser 110 erasmus mc fraction mutation effect basal 30 ° c analysis mouse addition domain buffer pseudosubstrate serine concentration type cgk ii 100 mm nacl cgk i ␣ ser 445 1 mm atp rat cgk ii activation cyclic nucleotide-stimulated autophosphorylation jonge amount region sequence level pseudosubstrate region study phosphate ser 114 incubation membrane ec 50 2.5 mm benzamidine 1 mm amp-pnp cyclic 32 p label function erasmu cgk ii activity cgk i ␤ pcpt-cgmp basal kinase activity radioactivity time periods www.jbc.org mutant acidic forms 8- pcpt-cgmp thr 109 hofmann phosphorylated residues phosphorylation sites autophosphorylated residues column phosphorylated forms november downloaded cgk i mercaptoethanol invitrogen library circle