Stoyanova, V.

fmr 1 promoter promoter fmr 1 gene fx cells methylated fusion page number citation purposes demethylation methylation cell lines cell fusion x syndrome allele number heterokaryon methylation status control cells control fibroblast purpose syndrome citation experiment clone panel status sample fx cell lines nuclei analysis demethylated study bisulfite reaction result unmethylated beads sequencing replication hfm cells genet dna replication region transcription cell population phase medium c 1 cells factor hypermethylated buffer dna demethylation mechanism population acids res marker 37° c expression snrpn genomic individual cpg island hybridization figure island mutation primer fx 1 cells pcr bias water bath 30 min 200 units semi-nested pcr procedure 95° c oostra volume demethylated status cell hybridization