Noort, J. van

rad 51 filament protein ammonium sulfate reaction recombination lament figure single-stranded dna structure double-stranded dna condition molecule length rad 51 fi presence sulfate fl ow cell ammonium disassembly recombinase double-stranded single-stranded acids research rad 51 protein formation assembly strand mg 2+ buffer exchange nucleotide activity nucleoprotein atp hydrolysis addition image mgcl 2 amp-pnp research hydrolysis force product nucleoprotein fi binding nucleotide cofactors experiment natl acad dna molecules fi lament molecule formation 30 min d-loop reaction conditions function puri fied 51. extension 37 c rad 51. monomer effect atpase rad 51 monomers fi lament structure protein –dna complexes rad 51 filaments fi lament disassembly scanning force microscopy fi lament assembly point figure 3 dna fragment strand exchange reca protein polymer appearance incubation recombination mediators 1.5 times