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igfbp igf-i level growth group kidney protein effect higfbp administration serum expression control factor insulin-like weight endocrinology animal study receptor binding snell dwarf mice hormone placebo hepatic system tubules journal growth hormone flyvbjerg value diabetes liver infusion henle change igf-i levels treatment kidney weight van neck mrna levels mouse primer epidermal growth factor increase dwarf igfbp -3 levels control group result adult insulin-like growth factor placebo control snell 7 days gh levels difference other gh secretion igf-i infusion group insulin-like growth factor-i region peritubular capillaries control values section insulin egf infusion igfbp -3 serum igf-i volume higfbp -1/ghra group ghbp mrna levels research staining secretion experiment kleffen amplicon size epidermal foundation method