Veen, S.W. van der

chondrocyte cartilage culture alginate collagen tissue redifferentiation density expansion growth factor matrix collagen type ii donor medium phenotype growth factors monolayer culture seeding engineering monolayer passage redifferentiation capacity articular study collagen type i seeding density amount articular chondrocytes capacity serum 11-fi brau ear chondrocytes experiment e 210 antibody seeding densities expression multiplication production number alginate beads graft result 11- redifferentiation culture beads van osch tissue engineering fibrau cartilage tissue engineering serum-free medium effect glycosaminoglycan sample staining extracellular matrix cytospin chondrocyte phenotype tissue engineering process condition expansion culture saturation density mouse 7500 cells /cm gag /dna production differentiation 11- fibrau ear cartilage serum-free cell numbers passaging university sigma dedifferentiation marker sca ffold igf 1 matrix components netherland