Griffioen, P.H.

sample kringle iv buffy coats kringle probe method blood digestion fragment buffy procedure genotyping lambda detection buffer leukocyte detection signal isolation genotype sensitivity serum concentration marker control sample study edta blood room temperature 7 min control boehringer mannheim genomic dna 2 min digestion buffer hybridization accuracy field gel electrophoresis kpn 1 digestion ladder bio-rad cell count wash step lipoprotein reaction blood samples signal electrophoresis chemistry trommsdorff number dextran result apolipoprotein mixture ii lambda markers ethidium bromide-stained gel elongation cycle buffy coat samples 7.5 105 cells modification 250 l 0.5 reproducibility nylon membrane lackner template dna centrifugation method genomic denaturation i lambda markers non-resolution zone plasma rotterdam hybridization mix 10 ml chemiluminescent detection protocol elongation step boehringer volume