http://repub.eur.nl/res/aut/1991/ List of Publications en http://repub.eur.nl/static-eur/img/logo.png http://repub.eur.nl http://repub.eur.nl/res/pub/31569/ 2010-12-01T00:00:00Z The concept of allocation of departure time slots is applied to alleviate and analyze road transport congestion. Demand is redistributed over departure time slots at on-ramps to analyze its relationship with the congestion in the network with minimized system travel time and optimized network utilization. The objective is to minimize the costs of early and late departure associated with any differences between the cumulative desired and optimum demand, with network capacity as a constraint. The genetic algorithm, a global-searching algorithm, is used to calculate the objective function. Then the consequences of the optimum demand for the traffic states are assessed through network simulation. The application of the approach of the A15 motorway in the Netherlands shows that early departure occurs for short journeys with low demand and late departure occurs mostly for long journeys with high demand. Moreover, the traffic state improves greatly with 7.0% reduction in total travel time and 8.9% reduction in congestion of temporal-spatial combinations. Authorities may use these results to take measures or make design policies that promote time-slot allocation, such as providing rewards for late or early departure outside the peak period. http://repub.eur.nl/res/pub/32836/ 2010-11-01T00:00:00Z Na/H exchanger regulatory factor 1 (NHERF1) is a scaffold protein made up of two PDZ domains and an ERM binding domain. It is in the brush border of multiple epithelial cells where it modulates 1) Na absorption by regulating NHE3 complexes and cytoskeletal association, 2) Cl secretion through trafficking of CFTR, and 3) Na-coupled phosphate absorption through membrane retention of NaPi2a. To further understand the role of NHERF1 in regulation of small intestinal Na absorptive cell function, with emphasis on apical membrane transport regulation, quantitative proteomic analysis was performed on brush border membrane vesicles (BBMV) prepared from wild-type (WT) and homozygous NHERF1 knockout mouse jejunal villus Na absorptive cells. Jejunal architecture appeared normal in NHERF1 null; however, there was increased proliferative activity, as indicated by increased crypt BrdU staining. LC-MS/MS analysis using iTRAQ to compare WT and NHERF1 null BBMV identified 463 proteins present in both WT and NHERF1 null BBMV of simultaneously prepared and studied samples. Seventeen proteins had an altered amount of expression between WT and NHERF1 null in two or more separate preparations, and 149 total proteins were altered in at least one BBMV preparation. The classes of the majority of proteins altered included transport proteins, signaling and trafficking proteins, and proteins involved in proliferation and cell division. Affected proteins also included tight junction and adherens junction proteins, cytoskeletal proteins, as well as metabolic and BB digestive enzymes. Changes in abundance of several proteins were confirmed by immunoblotting [increased CEACAM1, decreased ezrin (p-ezrin), NHERF3, PLCβ3, E-cadherin, p120, β-catenin]. The changes in the jejunal BBMV proteome of NHERF1 null mice are consistent with a more complex role of NHERF1 than just forming signaling complexes and anchoring proteins to the apical membrane and include at least alterations in proteins involved in transport, signaling, and proliferation. Copyright http://repub.eur.nl/res/pub/307/ 2001-10-04T00:00:00Z Labour flexibility in China???s Companies: An Empirical Study explores labour flexibility at the workplace in ten manufacturing companies in China. It addresses how HRM contributes and facilitates management in coping with increasing market competition. Flexible labour practices are related to the company level HR system, and change of the HR policies and practices entails a consistency perspective. Chinese managers have strong incentives to increase labour flexibility in the company. They simultaneously face constraints and/or opportunities as related to legacies and changes in the institutional framework of a Transition Economy.