Eekelen, C.C.E.M. van

lncap promoter activity lncap cells androgen receptor sequence expression psa promoter binding presence hormone absence r 1881 response lncap -1f cells effect lncap -1b region regulation element glucocorticoid fragment analysis psa promoter activity retardation method induction experiment position library erasmus mc incubation factor plasmid erasmu arr -2s growth transfection erasmus mc affinity antibody medium endocrinol concentration lncap cell line gel retardation analysis luciferase activity transfected psa -12-luc downloaded lncap -1f library january complex blot analysis gr expression study cell lines trapman activation luciferase subline 10 nm dex polyacrylamide gel electrophoresis gel retardation assay transcription protein psa gene level material room temperature non-prostate cell lines ar binding sites prostate clone tata box lncap cells overexpressing psa mrna expression gel retardation experiments androgen response region