Pathak, S.S.

affinity antibody concentration elisa response antigen method binding affinity distributions serum determination immuno sample distribution interaction value affinity maturation protein analysis plate equilibrium memory immunol chapter biosensor system assay equation maturation increase molecule dilution b cells surface mutation result enzyme antibody affinity reaction immunoglobulin measurement region center curve study mixture factor savelkoul ligand murine phase cuvette antigen-specific coating isotype affinity antibodies il -4 process hypermutation chain pathak sequential level polyclonal figure range hapten mouse change t cells mechanism effect specificity immobilization immunization nature substrate table conversion factor detection