Hatanaka, A.

/ cells ig gene conversion conversion sequence repair recombination i-scei embo j expression frequency donor endonuclease wild-type takeda chicken number nuclease i-scei site sonoda segment g 418-resistant colonies neor gene heterologous sequences sequence divergence hr frequency activity sister vector wild-type cells chicken fen phase chromatid takata colony sister chromatids double-strand transfected event gene conversion replication assay xpg / cells 2001. g 2 phase processing analysis 2002. efficiency divergence hr reactions result level ovalbumin locus saccharomyces cerevisiae nonhomologou structure reaction heterologou morrison overhang sensitivity substrate strand cerevisiae break material yeast japan dna ends i-scei-induced damage exo 1 i-scei expression vector nuclease expression vector function hr frequencies genetic i-scei-induced gene conversion 3 flap structure v 8 segment