Ramsden, D.A.

signal cleavage protein recombination hairpin substrate coding rag 1 sequence reaction rag 1 protein heptamer figure product rag 2 proteins strand formation hairpin formation rag 2 activity rag proteins position rag 2 protein flank nonamer coding ends cleavage reaction 12- mutation oligonucleotide spacer 12- signal gellert 23- 23- signal coding flank signal ends expression nicking recombination signal sequences experiment cleavage products signal sequences addition coding flank sequences phosphodiester bond recognition system extract structure species van gent sadofsky segment fraction condition electrophoresis signal sequence hesse recombination signal molecule result buffer column figure 2 recombination signals plasmid ramsden level fragment figure 2 b repair oettinger factor lmpcr apali baculoviru gelled m 12/23 rule nucleotide