McBlane, J.F.

signal cleavage protein recombination hairpin substrate coding rag 1 reaction sequence rag 1 protein figure product heptamer strand rag 2 proteins rag 2 formation position hairpin formation rag 2 protein activity cleavage reaction flank coding ends rag proteins nonamer oligonucleotide 12- 12- signal gellert 23- signal coding flank expression spacer recombination signal sequences mutation cleavage products 23- signal sequences addition coding flank sequences phosphodiester bond extract species van gent sadofsky fraction condition electrophoresis signal sequence signal ends experiment hesse recombination signal nicking buffer column figure 2 recombination signals plasmid level fragment figure 2 b system lmpcr apali baculoviru recognition structure molecule nucleotide cleavage activity hairpin species coding flank sequence vector bottom strand apali digestion phosphorylated 103/