Kakeno, M.

clasp clasp 2 iqgap 1 iqgap microtubule gsk -3β cos 7 cells phosphorylation antibody actin filaments egfp-clasp protein egfp-clasp 2-n ser 533 region actin gst-clasp cell biol ser 537 cell migration gst-clasp 2-n migration filament phosphorylated clasp 2 gsk -3β wt gsk -3 inhibitors material fig vero cells clip -170 result interaction phosphorylated fragment binding watanabe fukata assay clasp 2. gsk -3β. clasp 2-n -170 inhibitor regulation akhmanova kinase egfp-clasp 2 rac 1 transfected clasp 2 fragments serine /arginine region nonphosphorylated clasp 2 association control wittmann panel waterman-storer anti-gfp antibody japan sirna affinity material sds-page iqgap 1. clasp 2-binding region 1 hour microtubule sedimentation assays anti-s 533/s antibody 10 μ m science plus-end analysis phosphorylation sites effect phosphorylate c-terminal region lysate perinuclear region mbp-iqgap 1-ct mbp-iqgap expression