Vandekerckhove, B.

tcrgd population lineage t cells expression notch op 9 cells differentiation tcrgd þ cells op 9-dl cells analysis op 9-dl figure culture rearrangement 34highcd hematopoietic precursor t lineage cells blood pathway generation cd 34highcd cells cd thymocyte differentiation hesc-derived experiment tcrab phenotype number thymocyte leukemia dp cells representative receptor result van coppernolle co-culture tcra rearrangements thymu op 9-dl monolayers system marker stage mutation 34ϩcd tcrgd þ populations cd 34ϩ cells cd 34ϩcd cells mann – whitney t-cell op 9-gfp tcrab þ cells signi ficant differences stromal sample vandekerckhove structure von boehmer h hesc-derived t cells study fl ow cytometry university development t-all presence coculture dp pathway bd pharmingen -30 lymphoid stimulation coppernolle gammadelta signal level 9-gfp immunol cd 34highcd population tcra locus tcrgd þ t-all