Brande, J.V.L. van den

plasma growth growth hormone hormone activity cartilage factor study somatomedin assay fraction concentration daughaday effect patient tissue insulin serum sulfation factor ethanol protein sulfation acromegalic plasma radioactivity level material column acromegalic sulfate thymidine incorporation incubation response salmon result treatment technique uptake section hypophysectomized method hypophysectomized rats liver curve preparation value organ sephadex chromatography children acid ethanol extracts weight chapter medium acid ethanol substance animal procedure plasma sm extract kidney species gland difference ratio purification submandibular glands administration volume injection experiment incubation medium electrophoresis adrenal distribution somatomedine excretion blood ptf activity increase