Fraser, R.A.

sug 1 protein tfiih msug 1 transcription acids research yeast interaction hsug 1 fraction column buffer research antibody wild-type activity affinity 500 mm imidazole subunit mutation nature fusion analysis immunoprecipitation expression imidazole domain proteasome hoeijmaker xpb protein insect cells nickel-chelate affinity chromatography sequence vector binding repair immunoblot purification insect control function two-hybrid heparin weeda factor immunoblot analysis basal polymerase 100 mm imidazole xp 11be mutation vermeulen extract science addition transformant protein gsepharose xp 11be buffer b experiment chromatography protein asepharose fibroblast immunoblotting effect figure region component resin assay xpb cdna homolog tbp 1 finding complex peptide pa 700 helicase schaeffer lysate microinjection