Versteeg, G.A.

mouse protein isgylation virus isg 15 conjugation plasmid isg 15 conjugation b /ns mherc mherc 6 hherc herc 5 activity herc 6 domain mouse cells expression hherc 6 ligase mouse herc 6 transfected fluenza 293 t cells control enzyme system sirna ns 1 protein mouse isg 15 hherc 5 15. assay infection l -929 cells chimeric target antibody effect herc proteins type i ifn misg 15 transfection ubiquitin-like n-terminal herc 5/6 proteins e 3 ligase hisg 15 hek -293t cells mouse isg 15. target proteins encoding luciferase -929 january ifnb promoter mediate virol sinai school localization cell lysates issue sequence machinery e 2 enzymes mouse isgylation systems lysate www.plosone.org reporter volume n-terminal ubiquitin-like domain function speci v 5-specific immunoblot component experiment type i interferon result fluenza b viruses -293t