Kemp, L. van der

virus fluenza influenza sample sequence embryonated chicken eggs detection agarose gel electrophoresis dot blot hybridization hybridization virus strains pcr-based analysis oligonucleotide virus isolation culture isolation webster method result pcr analysis primer throat chicken strain dot blot analysis infection embryonated virus infection specimen assay pcr ampli fication position tmk cells segment stock cell cultures 10 min 0.2 ml pcr-based assays virus titers bird samples rna viruses human fluenza viruses agarose titer control panel cloacal fluenza virus primers primer sets netherland /duck table influenza virus primer sequences dot blots subtype transport medium electrophoresis virol background hybridization fication species pcr amplification sensitivity osterhau ampli virus nucleotide sequences diagnosis sequence information 0.02 tcid 50 influenza viruses virus stock virus sequences animal virus rna 0.2