Mensink, E.

patient quantitation tumor dilution probe myeloma tumor cells asopcr consensus variation sample product dilution assay sequence center coefficient assay blood variation coefficient multicenter marrow myeloma tumor cells pcr product vh 3fr probe number primers figure pcr intra pcr strategy igh asopcr consensus strategy intra value amount patient h cycle amplification myeloma patients result asopcr product polymerase reproducibility study method densitometric template treatment quantitate transplantation group primer chain patient samples calibration curve equations cell transplantation table reaction treatment response detection rearrangement tumor-specific region sensitivity clone taqman equation volume reference vh 3fr 50 cycles hematology plasma input dilution assays fluorescence leukemia fr 3 probes bone marrow cells calibration tumor cell concentrations